Doctors Data Functional Lab Testing | Advanced Specialty Testing | Tri-Health Wellness Centre Vaughan

Tri•Health

Specialty Laboratory Testing • Since 1972

Doctors Data Functional Lab Testing at Tri-Health

Advanced specialty testing that goes beyond standard blood work. Identify root causes of cardiometabolic risk, hormone imbalance, gut dysfunction, toxic burden, and nutritional deficiencies with clinically validated panels from Doctors Data — interpreted by our naturopathic physicians.

Discuss Testing Options Explore Panels

Why Specialty Testing

Beyond Conventional Lab Ranges

Standard blood panels often miss early dysfunction. Doctors Data specialty tests evaluate functional markers, metabolites, microbial ecology, toxic load, and genetic variants that influence how your body processes hormones, nutrients, and environmental exposures. At Tri-Health, results are interpreted in the context of your full clinical picture by Dr. Jason Granzotto ND and Dr. Maria Granzotto ND.

Cardiovascular & Metabolic Risk

CardioMetabolic Testing

Advanced lipid and inflammatory markers that refine cardiovascular risk assessment beyond total cholesterol and LDL alone.

CardioMetabolic Profile (Serum)

A comprehensive panel combining traditional lipids with advanced atherogenic and metabolic markers. It evaluates the full spectrum of lipid particles, inflammation, insulin dynamics, kidney filtration, and adipokine balance.

Key markers include: Adiponectin, Apolipoprotein A-1, Apolipoprotein B, hs-CRP, Cholesterol, Cystatin C, Glucose, GlycoMark, HDL, Homocysteine, Insulin, LDL, Leptin, Leptin/Adiponectin ratio, Lp(a), Oxidized LDL (OxLDL), PLAC (Lp-PLA2), Serum Creatinine, Triglycerides, sdLDL.

Clinical Conditions & Applications

Assessment of residual cardiovascular risk in patients with “normal” standard lipid panels
Metabolic syndrome, insulin resistance, and prediabetes
Family history of early heart disease or stroke
Monitoring response to lifestyle, dietary, or pharmacologic interventions
Evaluation of small dense LDL (sdLDL) and oxidized LDL particles that drive plaque formation

Elevated oxidized LDL has been shown in prospective studies to independently predict major adverse cardiovascular events in patients with type 2 diabetes and coronary heart disease, even after adjustment for conventional risk factors. Incorporating OxLDL and related markers can improve early identification of high-risk individuals.

Cardiovascular Risk Profile

Focused evaluation of atherogenic lipoproteins and inflammatory drivers of vascular disease. Ideal when the primary concern is plaque progression and residual risk rather than full metabolic assessment.

Includes: Apo A-1, Apo B, CRP, Cholesterol, HDL, Homocysteine, LDL, Lp(a), Non-HDL Cholesterol, OxLDL, PLAC, Triglycerides, sdLDL, vLDL.

Especially useful for: patients with elevated Lp(a), strong family history of CAD, or those seeking a more precise picture of particle number and oxidation status.

Metabolomic Profile & Oxidized LDL

The Metabolomic Profile examines adipokines (adiponectin, leptin), insulin, glucose dynamics (including GlycoMark for recent glycemic control), inflammation, and renal markers. Standalone Oxidized LDL testing isolates this highly atherogenic particle for targeted monitoring.

These panels help uncover early metabolic dysfunction that precedes overt diabetes or cardiovascular events, allowing proactive lifestyle and therapeutic strategies.

Hormone Balance & Neuroendocrine Health

Endocrinology & Hormone Testing

From comprehensive urinary hormone metabolites to diurnal salivary cortisol and neurotransmitter assessment — mapping the full steroid and neuroendocrine cascade.

Hormone and Urinary Metabolites Assessment Profile (HuMap™)

One of the most detailed non-invasive hormone evaluations available. HuMap measures parent hormones and an extensive array of phase 1 and phase 2 metabolites in urine, providing insight into production, metabolism, detoxification capacity, and oxidative stress.

Extensive markers include: Estrogens (E1, E2, E3) and metabolites (2-OH, 4-OH, 16-OH pathways and methoxy forms), progesterone and metabolites, androgens (T, DHT, DHEA/S, and downstream metabolites), cortisol and cortisone with diurnal pattern and tetrahydro metabolites, 8-hydroxy-2′-deoxyguanosine (oxidative stress), and calculated enzyme activity indicators (COMT, 11β-HSD, 5α-reductase, aromatase).

Conditions & Clinical Insights

Menopausal and perimenopausal symptoms, PMS/PMDD, endometriosis, PCOS
Hormone-related breast or prostate health concerns and family history of hormone-driven cancers
Fatigue, insomnia, mood changes, low libido, weight distribution changes
Monitoring of bioidentical hormone therapy (BHRT) and understanding how the body is processing supplemental hormones
Assessment of methylation capacity (COMT) and preferential estrogen detoxification pathways

Urinary hormone metabolite profiling provides unique information on phase 1 (hydroxylation) and phase 2 (methylation, conjugation) pathways that serum testing cannot fully capture. Preferential formation of certain catechol estrogen metabolites, when not adequately methylated, has been linked to increased oxidative DNA damage potential — information that can guide targeted nutritional and lifestyle support.

Neurotransmitter Profiles

Comprehensive Neurotransmitter and NeuroBasic Profile evaluate key neurotransmitters and metabolites in urine, offering a functional window into the balance of excitatory and inhibitory signaling.

Markers commonly include: Serotonin, 5-HIAA, Dopamine, DOPAC, Norepinephrine, Epinephrine, GABA, Glutamate, Glycine, Histamine, PEA, Taurine, Tyrosine, and related ratios.

Helpful in evaluating: mood disorders, anxiety, sleep disruption, focus and attention issues, chronic stress response, and suspected neurotransmitter imbalances contributing to fatigue or emotional dysregulation.

NeuroHormone Complete & Neuro Adrenal Panels

These combined panels integrate sex hormones, diurnal cortisol (Cx4), and key neurotransmitters. They are particularly valuable when symptoms span both endocrine and neurological domains — for example, fatigue with mood changes, or stress-related hormone disruption.

Adrenal & Diurnal Cortisol Testing

Multiple options allow precise mapping of the HPA axis:

Diurnal Cortisol Profile (Cx4) and Cortisol Awakening Response (CAR) — salivary collections that capture the natural daily rhythm and the dynamic morning rise.
Comprehensive Adrenal Function Profile — adds secretory IgA for mucosal immune insight.
Adrenal Corticoids (urine) — measures cortisol, cortisone, and their tetrahydro metabolites for a broader view of adrenal output and metabolism.

Salivary cortisol is a reliable, non-invasive tool for screening adrenal insufficiency and assessing circadian cortisol dynamics. Recent clinical evaluations confirm its utility for both diagnosis and monitoring of replacement therapy adequacy, with the ability to detect over- or under-replacement patterns across the day.

Commonly used for: chronic fatigue, burnout, sleep disruption, stress-related symptoms, suspected Cushing or Addison patterns, and optimization of adrenal support strategies.

Additional Hormone Options

Women’s Health & Breast Profile, Comprehensive Plus, Comprehensive Hormone, Short Comprehensive, and Basic Hormone profiles offer scalable depth depending on clinical need. Melatonin Profile (Mx3) evaluates the sleep hormone rhythm. Single or dual salivary hormone options allow targeted follow-up testing.

Gut Function & Microbiome

Gastrointestinal Health Testing

From inflammation and digestive enzyme markers to comprehensive microbiome mapping and pathogen detection — functional stool analysis that guides targeted gut restoration.

GI 360 & GI 360 Essentials

The GI 360 is among the most comprehensive stool evaluations available. It combines culture, PCR pathogen detection, parasitology, microbiome abundance profiling, short-chain fatty acid analysis, inflammation markers, digestive function indicators, and more.

Key components: Bacteriology & yeast culture, GI pathogen multiplex PCR, parasitology (concentrate + trichrome), microbiome abundance, calprotectin, lactoferrin, lysozyme, elastase, occult blood, short-chain fatty acids (acetate, butyrate, propionate, valerate), beta-glucuronidase, sIgA, pH, fat stain, muscle & vegetable fibers, mucus, WBC/RBC.

Clinical Applications

Chronic or recurrent digestive symptoms (IBS-like, bloating, irregularity, food sensitivities)
Inflammatory bowel disease monitoring and differentiation of inflammatory vs functional disorders
Suspected dysbiosis, SIBO overlap, or post-antibiotic recovery
Assessment of intestinal barrier integrity and secretory immune function
Evaluation of digestive enzyme output (elastase) and fat malabsorption

While consumer-grade microbiome tests show significant inter-lab variability, clinical-tier panels that include validated inflammation markers (calprotectin, lactoferrin), pancreatic elastase, and pathogen PCR retain established diagnostic utility. Research continues to refine how microbiome composition and short-chain fatty acid profiles relate to metabolic and immune health.

Comprehensive Stool Analysis ± Parasitology (CPP)

A robust foundational stool panel assessing bacteriology, yeast, inflammation, digestive markers, and (when ordered with parasitology) microscopic examination for parasites. The CPP adds multiplex PCR for common GI pathogens.

Useful for: unexplained GI symptoms, travel-related illness evaluation, chronic diarrhea or constipation patterns, and baseline gut assessment before deeper microbiome work.

Zonulin (Serum & Stool)

Zonulin is a key regulator of intestinal tight junctions. Elevated levels are associated with increased intestinal permeability (“leaky gut”).

Serum zonulin has been found elevated in both IBS-C and IBS-D compared with healthy controls, with levels comparable to those seen in active celiac disease in some cohorts. It correlates with stool frequency and bowel habit dissatisfaction in diarrhea-predominant IBS. Zonulin is also linked to metabolic syndrome, type 1 and type 2 diabetes risk, and various inflammatory conditions.

Consider when evaluating: suspected intestinal permeability, autoimmune conditions, food sensitivity patterns, metabolic dysfunction, and IBS.

Additional GI Markers & Add-ons

Calprotectin, Lactoferrin, Lysozyme, Elastase — inflammation and pancreatic function markers (available standalone or as add-ons)
H. pylori Antigen (stool) — non-invasive detection of Helicobacter pylori
Celiac & Gluten Sensitivity panels (blood spot or serum) including tTG, DGP, and gliadin antibodies
Short Chain Fatty Acids — acetate, butyrate, propionate, valerate assessment
Beta-Glucuronidase — enzyme activity relevant to estrogen and toxin recirculation
Secretory IgA — mucosal immune status
Yeast Culture, Bacteriology Culture, Comprehensive Clostridium Culture, Parasitology, Macroscopic Exam

Detoxification Capacity & Toxic Burden

Environmental Exposure & Detoxification

Assess genetic methylation capacity, oxidative DNA damage, glutathione status, hepatic detox markers, and porphyrin patterns that reflect toxicant effects on metabolic pathways.

DNA Methylation Profile (Buccal Swab)

Evaluates key single-nucleotide polymorphisms (SNPs) in genes that govern the methylation cycle and related pathways critical for DNA synthesis, neurotransmitter metabolism, detoxification, and hormone processing.

Genes commonly assessed: MTHFR (C677T, A1298C), MTR, MTRR, COMT, CBS, BHMT, AHCY, MAO-A, NOS, SHMT, SUOX, VDR, and others involved in one-carbon metabolism and related pathways.

Clinical Relevance

Understanding individual variation in folate and B12 metabolism
Homocysteine regulation and cardiovascular risk context
Neurotransmitter breakdown (COMT) and mood/stress response
Detoxification capacity and sensitivity to environmental exposures
Guidance for personalized nutrient support (forms and dosing of folate, B12, B6, betaine, etc.)

Methylation pathway variants, particularly in MTHFR and related genes, influence the efficiency of converting dietary folate into its active form and remethylating homocysteine. While not deterministic on their own, these genotypes help explain why some individuals require more targeted nutritional support and can inform safer, more effective supplementation strategies.

Hepatic Detox Profile, DNA Oxidative Damage & RBC Glutathione

Hepatic Detox Profile measures D-glucaric acid and mercapturic acids as indicators of Phase I and Phase II detoxification activity.

DNA Oxidative Damage Assay quantifies 8-hydroxy-2′-deoxyguanosine (8-OHdG), a widely studied marker of oxidative stress to DNA.

RBC Glutathione assesses intracellular levels of the body’s primary antioxidant and detoxification cofactor.

Helpful for: patients with high toxicant exposure history, chronic inflammation, fatigue, chemical sensitivity, or those optimizing detoxification pathways before or during therapeutic protocols.

Urine Porphyrins

Porphyrin patterns can reflect disruption of heme synthesis pathways by heavy metals and other toxicants. This profile evaluates multiple porphyrin fractions and ratios that may indicate specific toxic influences or metabolic blocks.

Heavy Metals & Mineral Status

Toxic & Essential Elements Testing

Multiple specimen types (blood, urine, hair, stool, RBC) allow evaluation of both recent and longer-term exposure as well as nutritional mineral status.

Blood, RBC, Serum, Urine, Hair & Stool Element Panels

Doctors Data offers a full suite of elemental testing:

Comprehensive Blood Elements / Whole Blood Elements — broad toxic and essential element assessment in whole blood
Red Blood Cell (RBC) Elements — intracellular mineral status (often more reflective of tissue levels than serum)
Serum Elements — key electrolytes and minerals
Urine Toxic & Essential Elements and Urine Toxic Metals — excretion patterns; useful with or without provocation
Hair Elements and Hair Toxic Element Exposure Profile — longer-term exposure trends
Stool Toxic Metals — gastrointestinal excretion of metals
Urine Mercury (standalone) and Creatinine Clearance

Common clinical contexts: unexplained neurological symptoms, fatigue, immune dysregulation, developmental or cognitive concerns, occupational or environmental exposure history, and optimization of mineral status in the context of chronic disease.

Nutrient Status & Metabolic Pathways

Nutritional Testing

Amino acid profiles, fatty acid balance, methylation intermediates, vitamin D status, and iodine/halide assessment.

Plasma & Urine Amino Acids

Comprehensive amino acid analysis reveals patterns related to protein intake and digestion, metabolic blocks, neurotransmitter precursors, detoxification capacity, and energy metabolism.

Extensive panel including essential and non-essential amino acids, intermediates (citrulline, ornithine, cystathionine, etc.), and related compounds.

Useful for: mood and cognitive concerns, fatigue, suspected protein maldigestion, methylation support needs, and personalized amino acid or protein recommendations.

Methylation Profile (Plasma) & Fatty Acids (Erythrocytes)

The plasma Methylation Profile measures key intermediates (homocysteine, methionine, SAM, SAH, cysteine, cystathionine) to assess functional methylation status beyond genetics alone.

Erythrocyte Fatty Acid analysis evaluates the balance of omega-3, omega-6, saturated, and monounsaturated fatty acids in red cell membranes — a longer-term reflection of dietary intake and metabolism relevant to inflammation, brain health, and cardiovascular risk.

Vitamin D, Iodine & Halides

Vitamin D (serum or blood spot) with D2/D3 differentiation. Urine iodine (with optional pre/post load) and halide panels (bromide, fluoride, iodine) assess status of these critical minerals and potential competitive inhibitors.

Find Why DNA

Targeted genetic markers related to metabolism, appetite regulation, and weight (including variants in FTO, MC4R, and others) that can inform personalized nutrition and lifestyle strategies.

Ready to Identify Root Causes?

Not every patient needs every test. At Tri-Health, Dr. Jason Granzotto ND and Dr. Maria Granzotto ND review your history, symptoms, and goals to recommend the most clinically relevant Doctors Data panels — then interpret results in the context of your whole-person care plan.

Book a Consultation Visit Tri-Health.ca

Doctor’s Data – Functional Testing